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Elisa data analysis excel4/9/2024 ![]() Test tubes or microfuge tubes capable of storing ≥1 mL Graph paper or computer software capable of generating or displaying logarithmic functions Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer Micropipettes with capability of measuring volumes ranging from 1 μl to 1 mL Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm) The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment: Ready-to-Use Substrate: 12 mL, Brown container.Wash Buffer (10x): 50 mL, Clear containter.400x Streptavidin-HRP: 30 μL, Blue container.Biotinylated Detection Antibody: Lyophilized, Yellow container.Protein Standard: Lyophilized (100 ng), Red container.Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips. ![]() The Human Carbonic Anhydrase IX ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Carbonic Anhydrase IX proteins within the range of 32-2000 pg/mL. Human: Carbonic Anhydrase XII, Carbonic Anhydrase XIV Murine: Carbonic Anhydrase IX The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross-reactivity or interference. The Human Carbonic Anhydrase IX ELISA is capable of recognizing both recombinant and naturally produced Human Carbonic Anhydrase IX proteins. The Human Carbonic Anhydrase IX ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Carbonic Anhydrase IX proteins. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Carbonic Anhydrase IX while the user-added detection antibodies bind to epitopes on the captured target protein. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The OmniKine? Human Carbonic Anhydrase IX ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Carbonic Anhydrase IX concentrations within any experimental sample including cell lysates, serum and plasma. Custom Recombinant Antibody (rAbs) Services.Annexin V-FITC Apoptosis Detection Kits.
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